Effect of Genotype and Explant Type on in Vitro Shoot Regeneration of Tomato (lycopersicon Esculentum Mill.)
نویسنده
چکیده
In order to select the best tomato cultivar for Agrobacterium mediated gene transformation studies, In vitro regeneration frequency of hypocotyls, leaf disc and shoot tip of five tomato cultivars (Lycopersicon esculentum) was investigated on a regeneration medium supplemented with 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. Significant differences in regeneration capacity between genotypes and explant types, expressed as frequency of regeneration, average number of callus, shoot primordial and regenerated shoot primordial were observed. Regeneration in 5 cultivars of tomato using 3 different explant types was achieved. The regeneration capacity was strongly influenced by the cultivar and explant type. The highest regeneration capacity was observed in cultivar Riograndea (80% by using shoot tip, 64.5% by using hypocotyl and 56% by using leaf disc) from all types of explant. Of the explant types shoot tip was found the best explant source for direct shoot formation (80% shoot primordial were regenerated) while hypocotyl was found the best explant source for shoot formation through callogenesis (64.5% shoot primordia were regenerated)
منابع مشابه
CALLUS INDUCTION, IN VITRO SHOOT REGENERATION AND HAIRY ROOT FORMATION BY THE ASSESSMENT OF VARIOUS PLANT GROWTH REGULATORS IN TOMATO (Solanum lycopersicum Mill.)
In vitro morphogenesis is greatly influenced by plant growth regulators (PGRs). The effect of different plant growth regulators was assessed on callus induction, in vitro shoot regeneration and hairy root formation in tomato. The maximum callus induction frequency was recorded culturing hypocotyls, while in vitro shoot regeneration frequency was significantly higher when leaf discs were used as...
متن کاملMolecular and biochemical protective roles of sodium nitroprusside in tomato (Lycopersicon esculentum Mill.) under salt stress
Salinity stresses act as inhibitor factors of plant growth. They can change the physiological characteristics and limit the production of crops. Sodium nitroprusside (SNP) is a stable free radical which use as a signalling molecule in plants and participates in various plant’s physiological, biochemical and molecular processes and also in plant’s responses to environmental stresses. We investig...
متن کاملEffect of Salinity Stress on Germination in Lycopersicon esculentum L. var Cal-ji
The response of tomato genotype Cal-ji against five salinity levels (distilled water as control, 25, 50, 75 and 100 mM) were studied at germination and early seedling stages. An experiment with conducted by using a completely randomized design (CRD) with three replications. Shoot and root length, shoot and root fresh weight, seed vigor, mean germination time, germination percentage an...
متن کاملIn vitro flowering and fruiting in tomato (Lycopersicon esculentum Mill.)
In vitro flowering and fruiting were induced in plants regenerated from calli of leaf explants of Lycopersicon esculentum var. Pant 11. MS medium supplemented with 2 mg/l BAP displayed the best response followed by MS with 2 mg/l BAP, 1 mg/ l ABA and 0.5 mg/l IAA. Flower buds were developed both directly from callus and regenerated plantlets from leaf explants under 16 h (39.3 μmol m-2s-1) phot...
متن کاملEffect of Salinity Stress on Germination in Lycopersicon esculentum L. var Cal-ji
The response of tomato genotype Cal-ji against five salinity levels (distilled water as control, 25, 50, 75 and 100 mM) were studied at germination and early seedling stages. An experiment with conducted by using a completely randomized design (CRD) with three replications. Shoot and root length, shoot and root fresh weight, seed vigor, mean germination time, germination percentage an...
متن کامل